Comparison of the Effect of Percoll Gradient and Swim- Up Sperm Preparation Techniques on Semen Parameters in Fertile And Infertile Males
Özet
Objective: The study aimed to compare the effects of Percoll gradient centrifugation and the swim-up method on semen parameters, including sperm concentration, motility, and morphology in fertile and infertile males to establishing which one between them can best improve the quality of sperm for Assisted Reproductive Technologies (ART).
Methods: This study was carried out in laboratory IVF Research and Application Center of Çukurova University Faculty of Medicine, Department of Obstetrics & Gynecology.Laboratory included 37 male patients divided into two groups which are fertile (Group I, n=20) and infertile (Group II, n=17) groups based on standard semen parameters. Sperm preparation was done using the swim-up technique and the Percoll gradient. Concentration, motility, and morphology taken - before and after preparation.
Results: Both methods significantly improved sperm quality. However, for the fertile group, compared to the swim-up method; this improvement in sperm motility and morphology was more pronounced in the case of the percoll gradient method. However, although both approaches advanced the quality of the sperm within infertile group but differences were not statistically important. In all groups except those who had infertility issues, there was substantial drop in the post-preparation sperm count but it was greater with respect to swim up among infertile male patients than in any other group.
Conclusion: Both techniques improve sperm quality; however, only slightly more changes were observed with respect to motility and morphology among fertile men when using the Percol gradient method. Therefore, it is suggested that, depending on the semen sample used; one might consider various types of methods for preparing sperm were considered to improve ART outcomes.
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